Evaluation of Oxacillin and Cefoxitin Disk Diffusion and Microbroth Dilution Methods for Detecting mecA -Mediated β-Lactam Resistance in Contemporary Staphylococcus epidermidis Isolates

Detection of Oxacillin Resistance in Staphylococcus aureus: Comparison of Phoenix Oxacillin and Cefoxitin MICs, MicroScan Oxacillin MIC, Oxacillin and Cefoxitin Disk Diffusion, and mecA Gene Detection

BACKGROUND. Phenotypic tests for detection of mecA containing staphylococci have been challenged. Cefoxitin (FOX) disk diffusion (DD) has been reported to more accurately predict mecA gene presence than oxacillin (OX) DD. The Phoenix instrument rapidly provides identification and susceptibility data and it is of interest to determine if an experimental panel containing OX and FOX could accurate...

Determination of vancomycin and methicillin resistance in clinical isolates of Staphylococcus aureus in hospitals of Ilam city

Introduction: In this study, using the phenotypic and genotypic methods, oxacillin susceptibility in Staphylococcus aureus (S. aureus) strains isolated from patients at two government hospitals in Ilam, Iran was tested. Materials and methods: Out of 200 S. aureus isolates from different human clinical specimens consisting of blood (31%), wound (20%), urine (21%), catheters (7%), sputum (12...

Identification and detection of methicillin resistance in non-epidermidis coagulase-negative staphylococci.

The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 microg) with oxacillin disks (1 microg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-n...

Evaluation of oxacillin and cefoxitin disk diffusion test for routine detection of methicillin resistant Staphylococcus aureus.

Detection Of Methicillin Resistance In Staphylococcus Aureus By Disk Diffusion And PCR Methods

  Background and Objective: Methicillin resistance in Staphylococcus aureus is an increasingly important clinical problem. A chromosomal gene, mecA, mediates resistance to penicillinase-resistant penicillins such as methicillin and oxacillin in Staphylococcus aureus. We evaluated the validity of disk diffusion test by using oxacillin, methicillin an...